Liposomal Nanoparticle Mediated Gene Modulation for the Treatment of Melanoma in the Murine Model

Authors

Bill Xu Tang BSA, Texas Tech University Health Sciences CenterFollow
Poonam Yadav PhD, The University of Texas MD Anderson Cancer CenterFollow
Rahul Anil Sheth MD, FSIR, The University of Texas MD Anderson Cancer CenterFollow

Files

Description

This study evaluated the efficacy of liposomal nanoparticles (LNPs) in delivering Wnt/β-catenin-inhibiting mIR-124 plasmids to melanomas. In vitro, LNPs with mIR-124 were applied to B16N2 melanoma cells. In vivo, LNPs were injected into B16N2 tumors in mice. Delivery success was confirmed via flow cytometry and RT-PCR, assessing GFP expression, tumor transition markers, and Wnt/β-catenin signaling. Overall, the findings suggested that LNP-mediated delivery of Wnt/β-catenin inhibitors holds promise as a therapeutic strategy for melanoma, with trends indicating a reversal of epithelial-mesenchymal transition (EMT) and slowed melanoma progression.

Program Affiliation

SOAR

Publication Date

2024

Keywords

Melanoma, Liposomal Nanoparticles (LNPs), Wnt/β-catenin Pathway, mIR-124

Recommended Citation

Tang, Bill Xu BSA; Yadav, Poonam PhD; and Sheth, Rahul Anil MD, FSIR, "Liposomal Nanoparticle Mediated Gene Modulation for the Treatment of Melanoma in the Murine Model" (2024). Students Summer in Oncology at Anderson Research (SOAR). 8.
https://openworks.mdanderson.org/soar24/8